Oligo
Oligo are described as the tiny, single-stranded DNA or even RNA molecules that comprises of a vast range of uses in genetic experimenting, study, as well as forensics. Generally created in the lab using the synthesis of solid-phase chemicals- the small pieces of nucleic acid are able to be manufactured using any kind of user-specified order, and therefore they are very crucial in the creation of artificial gene, polymerase chain reaction (PCR), DNA sequencing, molecular probes as well as library construction.
Purified/modified/special Oligo
Oligo that comprises of the common modifications like biotin, primary amines and fluorescein are able to be produced in all the levels of purification. For the case of Oligo that contain more multifaceted structures, HPLC purification is advised. This particular level of purification offers the optimal conditions necessary for the separation of full-length product because of the hydrophobicity conveyed by the glowing labels as well as the capability to choose an exceptional absorbance. There are various ways that can be used to create purified/modified/special oligo and they include; desalting, Reverse-phase cartridge purification (RP1), HPLC Reverse-phase, PAGE, Anion-Exchange HPLC, and Gel Filtration.
Fluorescent dyes
Fluorescent dyes consist of an eccentric property that enables them to absorb UV range as well as emit in visible range contained in the color spectrum. Every fluorescent dye contains its own exceptional properties that are used in several applications, such as in non-destructive testing, leak detection, antifreeze, water tracing, car wash soaps and detergents.
Hapten labels
Hapten labels can be described as labeled Nucleotides that are used in indirect detection techniques in collaboration with streptavidin or what is also known as antibody conjugates. To acquire heightened detection sensitivity, we advice for the use of tyramide signal amplification (TSA).
Modified bases
Modified bases or Nucleobases are biological compounds that contain nitrogen. They are also referred to as nitrogenous bases found inside nucleosides- the fundamental building blocks that are made of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA).